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Record 040 · Research · 2000
Measurement of antinuclear antibodies: assessment of different test systems
Clinical and Diagnostic Laboratory Immunology · requested inclusion
“Two independent sites compared rat-liver IIF, HEp-2 IIF, and ANA enzyme immunoassay (EIA) screening in 62 connective tissue disease patients and 398 controls. Agreement between HEp-2 and rat liver was 82.0% for positive/negative but only 51.0% for pattern.”
Two independent sites compared rat-liver IIF, HEp-2 IIF, and ANA enzyme immunoassay (EIA) screening in 62 connective tissue disease patients and 398 controls. Agreement between HEp-2 and rat liver was 82.0% for positive/negative but only 51.0% for pattern. Candidate notes: HEp-2 vs rat liver agreement: 82.0% (ANA positive/negative), 51.0% (ANA pattern) Between-site HEp-2 agreement: 71.8% for positive/negative, 86.5% for pattern Up to 98% of sera negative by ANA EIA but positive by rat liver/HEp-2 were from controls Limits noted in the record: Sera from a single German university hospital; consecutive routine-testing sample, not population-based Anti-ENA EIA applied only to a selected subset of sera A positive ANA is not a diagnosis. Claims in this record are not verified.
A positive ANA is not a diagnosis. Claims in this record are not verified.
source
- interpretation
- pattern
- testing-method
Research
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